Emmanuel Katto Dubai : Limiting the Number of Factors per Run in a DOE Design for Gene Screening

Hi everyone,

I’m Emmanuel Katto from Dubai, United Arab Emirates (UAE) working on screening 16 categorical factors (representing 16 different genes that I’m activating), but I’m facing a challenge. The total amount of DNA I can deliver is limited, so activating more than 8 genes per run leads to weaker activation, making it difficult to interpret the results. I need a way to limit the number of factors to 8 per run, but still capture the main effects and some 2-factor interactions (2FI) across all 16 genes.

Has anyone encountered this issue in a DOE design? Any strategies or approaches to achieve this would be greatly appreciated!

Thanks!
Regards
Emmanuel Katto